Great Western Woodlands, Leaf 13C Stable Isotope Data, Core 1ha, 2013 

The data set contains information on leaf ¹³C isotope composition studied on three species, Maireana sedifolia, Ptilotus obovatus and Eremophila scoparia from the core 1 ha Salmon Gum plot at the Credo, Great Western Woodland site. 

We at TERN acknowledge the Traditional Owners and Custodians throughout Australia, New Zealand and all nations. We honour their profound connections to land, water, biodiversity and culture and pay our respects to their Elders past, present and emerging. 

Data not provided. 

An analysis of stable isotopes for leaf traits was undertaken in the Salmon Gum plot at the Credo Great Western Woodland site. Leaves from three individuals from three species were collected and analyzed for carbon isotopic composition using a Picarro G2121-i Analyser.
Sampling and Analysis Methods: Leaf samples were collected from the SG100E 1ha Plot for analysis of the stable isotope ratio of δ¹³C. Collections of green leaves were gathered in 17/12/2013. Three plants of three species were sampled. A minimum of two leaves from three branches was sampled for each and kept in paper bags. Leaves were oven dried and then ground finely using a bead mill grinder and ~100- 200 mg of ground sample was transferred into a 3.5 mm X 5 mm tin capsule. The carbon isotopic composition was measured using a Picarro G2121-i Analyser for Isotopic CO₂.
The carbon isotope ratio of plant material (δ¹³C), is quantified on a per mil (‰) basis: δ¹³C = (R_sample / R_standard-1) X 1000 Where, R is the isotopic ratio of the sample or standard. R=δ¹³CO₂ / 12CO₂. The standard is represented by the carbon isotope ratio contained in a fossil belemnite from the Pee Dee limestone of South Carolina. Atropine and acetanilide were used as internal laboratory reference standards calibrated against international measurements standards: sucrose (IAEA-CH-6, δ¹³C VPDB = -10.45), cellulose (IAEA-CH-3, δ¹³C VPDB = -24.72) and graphite (USGS24, δ¹³C VPDB = -16.05). Values of δ¹³C in bulk leaf samples represent the degree of stomatal closure during the life of the leaf.