The data set contains information on leaf 13C isotope composition studied on three species, Maireana sedifolia, Ptilotus obovatus and Eremophila scoparia from the core 1 ha Salmon Gum plot at the Credo, Great Western Woodland site.
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An analysis of stable isotopes for leaf traits was undertaken in the Salmon Gum plot at the Credo Great Western Woodland site. Leaves from three individuals from three species were collected and analyzed for carbon isotopic composition using a Picarro G2121-i Analyser.
Sampling and Analysis Methods: Leaf samples were collected from the SG100E 1ha Plot for analysis of the stable isotope ratio of δ13C. Collections of green leaves were gathered in 17/12/2013. Three plants of three species were sampled. A minimum of two leaves from three branches was sampled for each and kept in paper bags. Leaves were oven dried and then ground finely using a bead mill grinder and ~100- 200 mg of ground sample was transferred into a 3.5 mm X 5 mm tin capsule. The carbon isotopic composition was measured using a Picarro G2121-i Analyser for Isotopic CO2.
The carbon isotope ratio of plant material (δ13C), is quantified on a per mil (‰) basis: δ13C = (Rsample / Rstandard-1) X 1000 Where, R is the isotopic ratio of the sample or standard. R=δ13CO2 / 12CO2. The standard is represented by the carbon isotope ratio contained in a fossil belemnite from the Pee Dee limestone of South Carolina. Atropine and acetanilide were used as internal laboratory reference standards calibrated against international measurements standards: sucrose (IAEA-CH-6, δ13C VPDB = -10.45), cellulose (IAEA-CH-3, δ13C VPDB = -24.72) and graphite (USGS24, δ13C VPDB = -16.05). Values of δ13C in bulk leaf samples represent the degree of stomatal closure during the life of the leaf.
Sampling and Analysis Methods: Leaf samples were collected from the SG100E 1ha Plot for analysis of the stable isotope ratio of δ13C. Collections of green leaves were gathered in 17/12/2013. Three plants of three species were sampled. A minimum of two leaves from three branches was sampled for each and kept in paper bags. Leaves were oven dried and then ground finely using a bead mill grinder and ~100- 200 mg of ground sample was transferred into a 3.5 mm X 5 mm tin capsule. The carbon isotopic composition was measured using a Picarro G2121-i Analyser for Isotopic CO2.
The carbon isotope ratio of plant material (δ13C), is quantified on a per mil (‰) basis: δ13C = (Rsample / Rstandard-1) X 1000 Where, R is the isotopic ratio of the sample or standard. R=δ13CO2 / 12CO2. The standard is represented by the carbon isotope ratio contained in a fossil belemnite from the Pee Dee limestone of South Carolina. Atropine and acetanilide were used as internal laboratory reference standards calibrated against international measurements standards: sucrose (IAEA-CH-6, δ13C VPDB = -10.45), cellulose (IAEA-CH-3, δ13C VPDB = -24.72) and graphite (USGS24, δ13C VPDB = -16.05). Values of δ13C in bulk leaf samples represent the degree of stomatal closure during the life of the leaf.
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