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Data

Archaeal Community Responses to a Decade of Ecological Restoration: OTU Raw Data Matrix 

Ver: 1
Status of Data: completed
Update Frequency: notPlanned
Security Classification: unclassified
Dataset Published: 2017-09-18
Dataset Last Modified: 2026-08-18
Metadata viewed 65 times
Dataset accessed 0 times
Metadata Created: 2022-10-18
Metadata Last Modified: 2026-08-19
How to cite this collection:
Gellie, N., Yan, D., Mills, J., Breed, M. & Lowe, A. (2017). Archaeal Community Responses to a Decade of Ecological Restoration: OTU Raw Data Matrix. Version 1. Terrestrial Ecosystem Research Network. Dataset. https://dx.doi.org/10.4227/05/59bf42700b048 
Data can be accessed from the following links:
HTTPOTU_Archea_raw
HTTPSample_sites_Mt_Bold_2
HTTPPoint-of-truth metadata URL
HTTPro-crate-metadata.json
We generated a total of 2,313,977 16S archaeal raw reads across the 36 replicates (64,277 ± 23,335 SD per replicate). A total of 2,299,955 archaeal sequences (63,888 ± 23,473 SD per replicate) and 1,937 archaeal OTUs (54 ± 20 SD per replicate) remained for further analysis after quality filtering. The OTU data provide information on archaeal flux at an active restoration site at Mt Bold, a water catchment reserve of the Mt Lofty Ranges in South Australia, through a stagger of years and can be used accordingly. 
Credit
We at TERN acknowledge the Traditional Owners and Custodians throughout Australia, New Zealand and all nations. We honour their profound connections to land, water, biodiversity and culture and pay our respects to their Elders past, present and emerging. We thank A. Bissett, S. Kennedy, Z. Baruch, S. Caddy-Retalic, L. Clarke, S. Kennedy, I. Fox, M. Laws, K. McCallum, and J. McDonald for technical and field assistance. We are grateful for support from the BASE project, Australian Genome Research Facility, BioPlatforms Australia, and SA Water. This work was supported by Australian Research Council funding to AJL and MFB (DE150100542; DP150103414), and China Scholarship Council funding to DY (201408410176). We would also like to acknowledge the contribution of the BASE project partners DOI 10.4227/71/ 561c9bc670099, an initiative supported by Bioplatforms Australia with funds provided by the Australian Commonwealth Government through the National Collaborative Research Infrastructure Strategy. 
Purpose
This project was conducted as a part of the author's PhD. The OTU data was generated for the manuscript titled "Archaeal community responses to a decade of ecological restoration" that uses eDNA assessment to provide a significant extension to current restoration monitoring practice. 
Lineage
Data not provided. 
Method DocumentationData not provided.
Procedure StepsData not provided.
Spatial Description
IBRA Region: Flinders Lofty Block
Our study system was an active restoration site at Mt Bold, a water catchment reserve of the Mt Lofty Ranges in South Australia (35.07;S, 138.42 E). This catchment was dominated by an open eucalypt woodland that has historically been subjected to tree clearance and grazing that began early in the 20th century. In this context, the native understory and most of the overstory was cleared and replaced by a grassland dominated by introduced grasses. At our study site, grazing ceased in 2003 when South Australia's water utility (SA Water) took over management. SA Water has actively restored the study site since 2005, with the restoration goal of recreating the local Eucalyptus leucoxylon dominated grassy woodland community. 
Temporal Coverage
From 2014-12-17 to 2014-12-18 
Spatial Resolution

Data not provided.

Vertical Extent

Data not provided.

ANZSRC - FOR
Conservation and biodiversity
Ecological applications
Environmental rehabilitation and restoration
Genetics
Microbiology
Plant biology
Soil sciences
Terrestrial ecology
Data Stream
Genomic
GCMD Sciences
ECOLOGICAL DYNAMICS - BIODIVERSITY FUNCTIONS
ECOLOGICAL DYNAMICS - ECOSYSTEM FUNCTIONS
ENVIRONMENTAL IMPACTS - CONSERVATION
RECLAMATION/REVEGETATION/RESTORATION
SOILS
Horizontal Resolution
1 km - < 10 km or approximately .01 degree - < .09 degree
Parameters
biological taxon name
latitude
longitude
operational taxonomic unit count
scientific name
Project
Others
Temporal Resolution
one off
Topic
biota
environment
User Defined
Biodiversity Inventory
Ecological Succession
Experimental Ecology
Landscape Ecology
Long-Term Community Monitoring
Long-Term Species Monitoring
Molecular Ecology
Restoration Ecology
Soil Ecology
Author
Gellie, Nicholas
Co-Author
Yan, Dongfeng
Mills, Jacob
Breed, Martin
Lowe, Andy
Contact Point
Gellie, Nicholas
Publisher
Terrestrial Ecosystem Research Network
A soil archaeal community responds to a decade of ecological restoration
By Parent record
Revegetation rewilds the soil bacterial microbiome of an old field- Part 1: OTU raw data matrix
Terrestrial Ecosystem Research Network
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(C)2017 University of Adelaide. Rights owned by University of Adelaide. 
Please cite this dataset as {Author} ({PublicationYear}). {Title}. {Version, as appropriate}. Terrestrial Ecosystem Research Network. Dataset. {Identifier}.