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Revegetation rewilds the soil bacterial microbiome of an old field- Part 1: OTU raw data matrix

Ver: 1.0
Status of Data: completed
Update Frequency: not planned
Security Classification: unclassified
Viewed: 0
Accessed: 0

Description

The authors analyzed a total of 3,002,411 quality-filtered bacterial 16S rRNA gene sequences in the 48 technical replicates across 8 revegetation chronosequence sites, consisting of 3,316 OTUs. Nine bacterial phyla dominated this dataset, including Acidobacteria, Actinobacteria, Bacteroidetes, Chloroflexi, Firmicutes, Gemmatimonadetes, Planctomycetes, Proteobacteria and Verrucomicrobia.The OTU data provide information on bacterial flux at this restoration site through a stagger of years and can be used accordingly.

Credit
We at TERN acknowledge the Traditional Owners and Custodians throughout Australia, New Zealand and all nations. We honour their profound connections to land, water, biodiversity and culture and pay our respects to their Elders past, present and emerging.
The authors thank A. Bissett, A. Fitzgerald, A. Young, Z. Baruch, S. Caddy-Retalic, L. Clarke, S. Kennedy, I. Fox, M. Laws, K. McCallum, and J. McDonald for technical and field assistance. We are grateful for technical, field and site access support from the Australian Genome Research Facility, BioPlatforms Australia, SA Water and the Terrestrial Ecosystem Research Network. This work was supported by Australian Research Council funding to AJL and MFB (DE150100542; DP150103414).

Purpose
This project forms part of the authors PhD. The OTU data was generated for a manuscript in Molecular Ecology "Revegetation rewilds the soil bacterial microbiome of an old field" https://doi.org/10.1111/mec.14081 that uses eDNA assessment to provide a significant extension to current restoration monitoring practice.

Lineage
EDNA metabarcoding: Three 25 m x 25 m quadrats were randomly selected per site, giving a total of 24 quadrats across the 8 sites. Soil was sampled from the 0-10 cm and 20-30 cm soil horizons at each quadrat. A representative 50 g sample of soil was collected at each of these 24 quadrats by pooling nine soil samples from each soil depth, including soil from open areas and under plants. These nine soil samples were pooled into a sterile plastic bag, and homogenised using a sterilised trowel. All soil samples (n = 48) were frozen on site in sterile 50 mL falcon tubes until DNA extraction- hereafter referred to as technical replicates.

Temporal coverage

From 12/17/2014 to 12/18/2014

Dates

Date modified

1/13/2017

Citation information

How to cite this collection:

Gellie, N., Mills, J., Breed, M. & Lowe, A. (2017): Revegetation rewilds the soil bacterial microbiome of an old field- Part 1: OTU raw data matrix. Version 1.0. Terrestrial Ecosystem Research Network. (Dataset). https://doi.org/10.4227/05/5878480a91885

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Rights and Licensing

CC-BY

Creative Commons Attribution 4.0 International Licence
http://creativecommons.org/licenses/by/4.0

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Please cite this dataset as {Author} ({PublicationYear}). {Title}. {Version, as appropriate}. Terrestrial Ecosystem Research Network. Dataset. {Identifier}.
(C)2017 University of Adelaide. Rights owned by University of Adelaide.

Keywords

Parameters
GCMD Science
ANZSRC - FOR
Horizontal Resolution
Vertical Resolution
Temporal Resolution
Topic Categories
User Defined
  • Biodiversity Inventory
  • Carbon Dynamics
  • Ecological Succession
  • Eucalypt Open Woodlands
  • Eucalypt Woodlands
  • Experimental Ecology
  • Landscape Ecology
  • Long-Term Community Monitoring
  • Long-Term Species Monitoring
  • Microbial Ecology
  • Modified Native Vegetation
  • Molecular Ecology
  • Restoration Ecology
  • Revegetation
  • Soil Ecology
  • Soil microbiome

People and Organisation

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Data Quality

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Contacts
  • Terrestrial Ecosystem Research Network
  • Building 1019, 80 Meiers Rd
  • QLD 4068
  • Australia
Spatial coverage
Spatial description
The study system was an active restoration site at Mt Bold, a water catchment reserve of the Mt Lofty Ranges in South Australia (35.07;S, 138.42 E). This catchment was dominated by an open eucalypt woodland that has historically been subjected to tree clearance and grazing that began early in the 20th century. In this context, the native understory and most of the overstory was cleared and replaced by a grassland dominated by introduced grasses. At the study site, grazing ceased in 2003 when South Australia's water utility (SA Water) took over management. SA Water has actively restored the study site since 2005, with the restoration goal of recreating the local Eucalyptus leucoxylon dominated grassy woodland community.