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Data

High-throughput eDNA Monitoring of Fungi to Track Functional Recovery in Ecological Restoration: OTU Raw Data Matrix 

Ver: 1
Status of Data: completed
Update Frequency: notPlanned
Security Classification: unclassified
Dataset Published: 2017-03-16
Dataset Last Modified: 2026-08-18
Metadata viewed 184 times
Dataset accessed 3 times
Metadata Created: 2022-10-25
Metadata Last Modified: 2026-08-19
How to cite this collection:
Gellie, N., Yan, D., Mills, J., Lowe, A. & Breed, M. (2017). High-throughput eDNA Monitoring of Fungi to Track Functional Recovery in Ecological Restoration: OTU Raw Data Matrix. Version 1. Terrestrial Ecosystem Research Network. Dataset. https://dx.doi.org/10.4227/05/58ca32e5ef782 
Data can be accessed from the following links:
HTTPFungi_OTU
HTTPSample_sites_MtBold_fungi
HTTPPoint-of-truth metadata URL
HTTPro-crate-metadata.json
We present a High-throughput eDNA dataset of fungi to track functional recovery in ecological restoration in the form of an OTU raw data matrix. We generated a total of 4,993,144 ITS fungal raw reads (118,884 ± 42,210 SD per replicate) across the 42 replicates. A total of 4,955,680 fungal sequences (117,430 ± 42,164 SD per replicate) remained for further analysis after quality filtering. The OTU data provide information on fungal flux at this restoration site through a stagger of years and can be used accordingly. 
Credit
We at TERN acknowledge the Traditional Owners and Custodians throughout Australia, New Zealand and all nations. We honour their profound connections to land, water, biodiversity and culture and pay our respects to their Elders past, present and emerging. We thank A. Bissett, S. Kennedy, Z. Baruch, S. Caddy-Retalic, L. Clarke, S. Kennedy, I. Fox, M. Laws, K. McCallum, and J. McDonald for technical and field assistance. We are grateful for support from the BASE project, Australian Genome Research Facility, BioPlatforms Australia, and SA Water. This work was supported by Australian Research Council funding to AJL and MFB (DE150100542; DP150103414), and China Scholarship Council funding to DY (201408410176). We would also like to acknowledge the contribution of the BASE project partners DOI 10.4227/71/ 561c9bc670099, an initiative supported by Bioplatforms Australia with funds provided by the Australian Commonwealth Government through the National Collaborative Research Infrastructure Strategy. 
Purpose
This project was conducted during the author's PhD. The OTU data was generated for the manuscript titled "High-throughput eDNA monitoring of fungi to track functional recovery in ecological restoration" that uses eDNA assessment to provide a significant extension to current restoration monitoring practice. 
Lineage
Data not provided. 
Method DocumentationData not provided.
Procedure StepsData not provided.
Spatial Description
Our study system was an active restoration site at Mt Bold, a water catchment reserve of the Mt Lofty Ranges in South Australia (35.07;S, 138.42 E). This catchment was dominated by an open eucalypt woodland that has historically been subjected to tree clearance and grazing that began early in the 20th century. In this context, the native understory and most of the overstory was cleared and replaced by a grassland dominated by introduced grasses. At our study site, grazing ceased in 2003 when South Australia's water utility (SA Water) took over management. SA Water has actively restored the study site since 2005, with the restoration goal of recreating the local Eucalyptus leucoxylon dominated grassy woodland community. 
Temporal Coverage
From 2014-12-17 to 2014-12-18 
Spatial Resolution

Data not provided.

Vertical Extent

Data not provided.

ANZSRC - FOR
Conservation and biodiversity
Environmental management
Environmental rehabilitation and restoration
Genetics
Microbiology
Plant and fungus systematics and taxonomy
Terrestrial ecology
Data Stream
Genomic
GCMD Sciences
ECOLOGICAL DYNAMICS - BIODIVERSITY FUNCTIONS
FUNGI
RECLAMATION/REVEGETATION/RESTORATION
Horizontal Resolution
1 km - < 10 km or approximately .01 degree - < .09 degree
Parameters
latitude
longitude
operational taxonomic unit count
scientific name
Project
Others
Temporal Resolution
one off
Topic
biota
User Defined
Biodiversity Inventory
Ecological Succession
Experimental Ecology
Landscape Ecology
Long-Term Community Monitoring
Long-Term Species Monitoring
Molecular Ecology
Restoration Ecology
Soil Ecology
Author
Gellie, Nicholas
Co-Author
Yan, Dongfeng
Mills, Jacob
Lowe, Andy
Breed, Martin
Contact Point
Gellie, Nicholas
Publisher
Terrestrial Ecosystem Research Network
High-throughput eDNA monitoring of fungi to track functional recovery in ecological restoration
By Parent record
Revegetation rewilds the soil bacterial microbiome of an old field- Part 1: OTU raw data matrix
Terrestrial Ecosystem Research Network
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Please cite this dataset as {Author} ({PublicationYear}). {Title}. {Version, as appropriate}. Terrestrial Ecosystem Research Network. Dataset. {Identifier}. 
(C)2017 University of Adelaide. Rights owned by University of Adelaide.