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Fletcherview Tropical Rangelands Soil Environmental DNA (eDNA) Dataset 

Ver: 1
Status of Data: completed
Update Frequency: notPlanned
Security Classification: unclassified
Dataset Published: 2022-08-01
Dataset Last Modified: 2026-08-18
Metadata viewed 353 times
Dataset accessed 73 times
Metadata Created: 2022-07-26
Metadata Last Modified: 2026-08-19
How to cite this collection:
Edwards, W. (2022). Fletcherview Tropical Rangelands Soil Environmental DNA (eDNA) Dataset. Version 1. Terrestrial Ecosystem Research Network. Dataset. https://portal.tern.org.au/metadata/ee08bdaf-28b7-4b15-9f74-6fc1acd6b039 
Data can be accessed from the following links:
HTTPSoil eDNA Data Files
HTTPFile naming
HTTPPoint-of-truth metadata URL
HTTPro-crate-metadata.json
This dataset contains soil microbial and genomic analysis files of 9 soil samples from each of three plots at Fletcherview, Northern Queensland (NQ) processed by the Australian Genome Research Facility Ltd (AGRF) . The files are available as compressed FastQ formatted sequence files.
For the nine Far North Queensland (FNQ) new plots (3 plots in Fletcherview and six plots at Wambiana), soil sampling additional to that done as component of plot installation by TERN have been undertaken. This is aligned with potential future exploratory work on soil eDNA proposed for WA. The protocol is a modified version of the BASE sampling protocol, combined with soil sampling as per White et al. (2012).
DNA extracted from the soil samples and Metagenomics 10Gbp (giga base pairs) bundle as per AGRF protocol. 
Credit
We at TERN acknowledge the Traditional Owners and Custodians throughout Australia, New Zealand and all nations. We honour their profound connections to land, water, biodiversity and culture and pay our respects to their Elders past, present and emerging. This work was jointly funded by the Terrestrial Ecosystem Research Network (TERN), an Australian Government National Collaborative Research Infrastructure Strategy (NCRIS) project, and the Queensland Government Research Infrastructure Co-investment Fund (RICF). 
Purpose
This is aligned with potential future exploratory work on soil eDNA. Resolve microbial diversity for the TERN Fletcherview site (3 plots), plus provide reference sequence collection to assess ability to detect ant species collected at same time and location. Used in biodiversity monitoring assessment. Ants identified by Alan Anderson (CDU) and sequenced. Also allow test of ability to detect vertebrates from eDNA using vertebrate sequences collected by Prof Lin Schwarzkopf (JCU). 
Lineage
Data not provided. 
Method DocumentationData not provided.
Procedure StepsData not provided.
Spatial Description
Plots QDAEIV0008; QDAEIV0009, QDAEIV0010 
Temporal Coverage
From 2021-10-19 to 2021-10-19 
Spatial Resolution

Data not provided.

Vertical Extent

Data not provided.

ANZSRC - FOR
Sequence analysis
Soil sciences
Data Stream
Genomic
GCMD Sciences
ANIMALS/INVERTEBRATES
SOILS
Horizontal Resolution
1 meter - < 30 meters
Instruments
Illumina NovaSeq 6000
Parameters
gene family abundance
sequencing reads
Platforms
Fletcherview Tropical Rangeland, Black soil
Fletcherview Tropical Rangeland, Core1ha
Fletcherview Tropical Rangeland, Red basalt
Project
TERN Ecosystem Processes
Temporal Resolution
one off
Topic
biota
environment
User Defined
genomics
metabarcoding
soil biodiversity
species presence
Vertical Resolution
< 1 meter
Author
Edwards, Will
Contact Point
Edwards, Will
Publisher
Terrestrial Ecosystem Research Network
Terrestrial Ecosystem Research Network
80 Meiers Road, Indooroopilly, Queensland, 4068, Australia.
Contact Us
Creative Commons Attribution 4.0 International Licence
https://creativecommons.org/licenses/by/4.0/
The Creative Commons Attribution 4.0 International (CC BY 4.0) license allows others to copy, distribute, display, and create derivative works provided that they credit the original source and any other nominated parties. Details are provided at https://creativecommons.org/licenses/by/4.0/ 
TERN services are provided on an "as-is" and "as available" basis. Users use any TERN services at their discretion and risk. They will be solely responsible for any damage or loss whatsoever that results from such use including use of any data obtained through TERN and any analysis performed using the TERN infrastructure.
Web links to and from external, third party websites should not be construed as implying any relationships with and/or endorsement of the external site or its content by TERN.

Please advise any work or publications that use this data via the online form at https://www.tern.org.au/research-publications/#reporting 
Please cite this dataset as {Author} ({PublicationYear}). {Title}. {Version, as appropriate}. Terrestrial Ecosystem Research Network. Dataset. {Identifier}.